Visualisation of Microscopic Images
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Visualisation of Microscopic Images |
Procedimentos |
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Procedures:
Cut the word "forma" from the newspaper (1cm2) or write it in square pieces of paper (1cm2);
Put one drop of water over the slide;
Put the word "forma" on the slide and drop one drop of water over the paper (figures 1a and 1b);
Remove the excess of water with the filter paper (figures 1c and 1d);
Elevate the body tube and the objective lens with the macrometric knob (figure 1e);
Place the slide (preparation) in the chariot or clips (figure 1f);
Light the microscopic field with the slide or mirror deviation (figure 1g);
Looking from the outside, turn the revolver until you have the objective lens with the least magnification;
Still looking from the outside, in order not to break the slide, turn the macrometric knob until the objective lens is near the slide;
Looking through the ocular lens, slowly turn the macrometric knob for the objective lens to go away from the slide until you have an image (figure 1h);
Turn the micrometric knob to focus the image;
To move to a higher magnification degree, repeat the operation with a greater magnification objective lens.
Figure 1: Assembly steps and visualisation of the specimen in the microscope
Occurrence:
The greater magnification objective lens allows visualisation of a smaller area;
The lower magnification objective lens allows visualisation of a larger area.
From the word selected only the larger syllabus was seen;
Letter "f" was seen inverted and repelled;
Letter "r" was seen inverted and repelled;
Letter "O" apparently was not seen inverted or repelled.
Procedures with the word "FEIRA":
The same procedures conducted for the word "forma" (figure 2);
Figure 2: Assembly steps and visualisation of the specimen in the microscope, and the result of the observation of the preparation under different magnification grades.
Conclusions:
The use of the greater magnification objective lens, as it allows visualisation of a smaller area, allows observation of details in the preparation in question.
The use of the lower magnification objective lens, as it allows visualisation of a larger area, allows observation of few details in the preparation in question;
Changes in the position of the letters were noted.